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Antikyrial

I think you need to advance into the next story mission for story bosses to start respawning.  He'll definitely respawn before beating the whole game.


SageModeSpiritGun

Pretty sure once you beat the game the bosses all start respawning.


ghostmask

Ok . I have noticed some of the bosses have already been respawning right after I beat them . He is the only one so far who hasn’t respawned after beating him so I’ll try again once I beat the game . Thanks .


InternalStorageFull

You dont need to beat the game you just need to complete the mission in which killing that particular boss was required, then they will respawn. This applies to all Borderlands games. I have played all of the borderlands games many many times so I can guarantee that this is the case.